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human astrocytes cell line

WebHuman Normal Astrocyte Cell Line Svgp12, supplied by ATCC, used in various techniques. WebHuman primary cells more closely mimic the physiological state of cells in vivo and generate more relevant data representing living systems. By using this website, you agree to our Characteristics of differentially expressed proteins in SVG P12 cells. You can update your default account on the My Dashboard Accounts Management page at any time. Yinfei Wu carried out most of the experiments. PVDF membrane was used for transmembrane. The absorbance of each hole in the 96-well plate was measured by enzyme-labeled instrument at 562nm wavelength. and transmitted securely. 2021;2(1):3. We conclude that methods based on the use of SV40 T antigen can successfully immortalize human astrocytes, retaining key astrocyte functions, but T antigen-induced proliferation appeared to interfere with expression of glial fibrillary acidic protein. PubMed Central Astrocyte-endothelial interactions and blood-brain barrier permeability. By activating astrocytes, tumor cells adapt to the new microenvironment and begin to colonize and migrate. Animal 2014;156:100216. We nurture, reward and retain the top talents with the best resource to build a strong team. Molecular functions related include NAD binding, electron transfer activity, ATP binding, and protein kinase regulator activity. Detection of cytokines secreted by SVG P12 cells in each group by Luminex liquid suspension chip. Exo: SVG P12 cells co-cultured with H1299 cell-derived exosomes for 24h). 2012;21(5):64254. 2022 May 13;25(6):104404. doi: 10.1016/j.isci.2022.104404. Clipboard, Search History, and several other advanced features are temporarily unavailable. Incomplete - you did not complete your application online, Out for signature - the signature process is not complete, Under review - ATCC is currently reviewing your application. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Kitamura K, Ito R, Umehara K, Morio H, Saito K, Suzuki S, Hashimoto M, Saito Y, Anzai N, Akita H, Chiba K, Furihata T. J Pharmacol Sci. 7). The structure of blood-tumor barrier and distribution of chemotherapeutic drugs in non-small cell lung cancer brain metastases. KEGG pathway annotation and KEGG enrichment analysis showed that differentially expressed proteins could participate in metabolic pathways regulation, biosynthesis of secondary metabolites, pathways of neurodegeneration-multiple disease, endocytosis, PI3K-Akt signaling pathway, MAPK signaling pathway, and so on (Fig. Umehara K, Sun Y, Hiura S, Hamada K, Itoh M, Kitamura K, Oshima M, Iwama A, Saito K, Anzai N, Chiba K, Akita H, Furihata T. Mol Neurobiol. Zhezhi Deng et al. Creative Biolabs is dedicated to serving life science research communities worldwide through global operation networks. After incubating Edu working solution with each group of cells for 2hours, 4% paraformaldehyde was added for cell fixation at room temperature for 15minutes. Tumor-derived exosomes drive pre-metastatic niche formation in lung via modulating CCL1+fibroblast and CCR8+ Treg cell interactions. Primary human astrocytes produce 24(S),25-epoxycholesterol with implications for brain cholesterol homeostasis. ( A ) Schematic overview of the genomic organization of Tha-eGFP and Th2P-4M-eGFP. We will contact you as soon as possible. sharing sensitive information, make sure youre on a federal Development of a three-dimensional blood-brain barrier network with opening capillary structures for drug transport screening assays. Creative Biolabs will use your email address to confirm your identity and send you transaction-related emails, promotional and customer service emails in accordance with our privacy policy. Laboratory research use only (RUO). 2, the activity of SVG P12 cells gradually declined with the increase of exosome concentration, suggesting that tumor-cell-derived exosomes could affect the activity of astrocytes. After 24hours of cultivation, the cell supernatant was collected into a 50ml centrifuge tube. 2007 Jan;210(1):54-65. doi: 10.1111/j.1469-7580.2006.00671.x. Part of 2020;12(12):1178193. Keep up to date with our events, news, and more. 2015;160(3):393406. WebThe ATCC collection has many neural cell lines, representing the normal and diseased tissue of multiple species such as neurons and the supporting cells of the central and 1.6.14.0) was applied to search the protein database, and LFQ (LabelFreeQuantity) algorithm was used to conduct the protein quantitative analysis. You have previously started an account application. Disclaimer. When co-cultured with neuronal cells, HASTR/ci35 cells could facilitate their dendritic network formation. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Klein A, Schwartz H, Sagi-Assif O, et al. Epub 2017 Nov 11. We will contact you as soon as possible. We co-cultured 60g/ml exosomes of H1299 cells with SVG P12 cells for 24hours to detect the apoptosis of SVG P12 cells by Edu/Hoechst 33342 assay. In Vitro Cellular & Developmental Biology. 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of Calcium Imaging Technology in Nervous System Research, Applications of Optogenetics in Rodents and Non-human Primates. 2021;33(10):20402058.e10. Reveal that CBX6 overexpression inhibits proliferation of glyoblastoma cells and. P<0.05 was considered a statistical difference. When devoid of GRN, mbOrgs spontaneously recapitulate TDP-43 mis-localization, hyperphosphorylation, and LoF phenotypes. Google Scholar. Extracellular metabolic energetics can promote cancer progression. For more information, please browse the options below to take a deeper look at the tools for your research needs. The film transfer device was installed, and the film was transferred on the ice with a constant current of 280mA and a time of 90min. It is well known that metastatic tumor cells require specific energy, nutrient, and oxygen patterns to compete with the in-situ cells at the site of metastases so that they could adapt to the local tissue microenvironment and establish metastatic colonies. The .gov means its official. Exosomes of H1299 could induce apoptosis of astrocytes as well as promote their secretion of cytokines that were conducive to the formation of the inflammatory microenvironment and immunosuppressive microenvironment, and affect their metabolic pathways, thus facilitating the formation of pre-metastatic niche in lung cancer brain metastases. This study proved for the first time that the exosomes secreted by lung cancer cell line H1299 could significantly change the expression of apoptosis related proteins MAP2K1, TUBA1C, RELA, and CASP6, thus promoting apoptosis of human astrocyte line SVG P12 cells. Furthermore, HASTR/ci35 cells not only possessed significant glutamate and adenosine transporter activities but also exhibited organic ion transporter activities. DMEM [Glucose, L-Glutamine, Sodium Pyruvate], CocKit Endothelial Cell Growth Medium [MV Microvascular Endothelial Cells], CocKit Endothelial Cell Growth Medium [Endothelial Cells], Supplements and Growth Factors Kit [MV Microvascular Endothelial Cells], Supplements and Growth Factors Kit [Endothelial Cells], Endothelial Cell Growth Basal Medium-2, 500 mL, Retinal Epithelial Cell Growth Medium Supplements and Growth Factors, CocKit Retinal Pigment Epithelial Cell Growth Medium Kit, CocKit Primary Neuron Growth Medium [Adult], Primary Neuron Growth Medium Supplements and Growth Factors [Adult], Primary Neuron Growth Medium Supplements and Growth Factors, Rat Cardiomyocyte Growth Medium Supplements and Growth Factors, CocKit Rat Cardiomyocyte Growth Medium (rCGM) Kit, Astrocyte Growth Medium Supplements and Growth Factors, Mouse BALB SFME (Serum Free Mouse Embryo) Cells, Mouse C8-S [Astrocyte type II clone] Cells, Mouse C8-D30 [Astrocyte type III clone] Cells, Human Brain Microglia Cells - Immortalized, GFP Expressed, iNeu Human Neural Stem Cells, Cryopreserved. The BBB is composed of endothelial cells, pericytes, and astrocytes [8]. The results showed that CD9 and TSG 101 proteins were positively expressed on exosomes, while Calnexin proteins were negatively expressed. WebAstrocyte Cell Lines Astrocytes are the most abundant, numerous, and diverse type of glial cells in the central nervous system. Cryopreserved NHA are shipped as frozen primary-derived cultures. Normal Human Astrocytes (NHA)can be used to study the function of the central nervous system and how neural cells interact. Chang ML, Wu CH, Jiang-Shieh YF, Shieh JY, Wen CY. Please see the material transfer agreement (MTA) for further details regarding the use of this product. Generation of a Human Conditionally Immortalized Cell-based Multicellular Spheroidal Blood-Brain Barrier Model for Permeability Evaluation of Macromolecules. HA are cryopreserved at passage one and delivered frozen. Human Astrocytes Transfer Aggregated Alpha-Synuclein via Tunneling Nanotubes Many lines of evidence suggest that the Parkinson's disease (PD)-related Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. WebThis review explores the interface between circadian timekeeping and the regulation of brain function by astrocytes. Unauthorized use of these marks is strictly prohibited. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. J Neuroimmunol. Int J Mol Sci. You've successfully associated your account with your Profile. 3D, E). PubMed: 6466977, Alliot F, et al. Biochemical pharmacology. Carcinogenesis. In vitro astrocyte models are valuable experimental tools in various astrocyte studies. For example, Tominaga et al. This item is part of a JSTOR Collection. The vertical axis showed the name of each GO function, and the value in horizontal axis was the number of differentially expressed proteins contained in that function. HNSC.100 is a clonal cell line established from neural progenitor cells from human fetal brain tissue transduced with a v-myc expression vector [ 18 ]. The name of each KEGG pathway was displayed in the vertical axis, and the value in horizontal axis was the P value after -log10 conversion. WebAstrocyte endfeet enwrap the entire vascular tree within the central nervous system, where they perform important functions in regulating the blood-brain barrier (BBB), cerebral blood flow, nutrient uptake, and waste clearance. Before WebPrimary human astrocytes were transfected with the SV40 T antigen expression vectors, pSV3neo or p735.6, and cultures were established with an extended lifespan. Astrocytes are the most widely distributed and abundant glial cells in the brain, which are involved in maintaining BBB characteristics and the stability of the brain microenvironment [9]. Effects of H1299 cell-derived exosomes on apoptosis of SVG P12 cells. 2007;13(6):166374. 2021 May;200:101973. doi: 10.1016/j.pneurobio.2020.101973. Creative Biolabs has been dedicated to serving life science research clients worldwide through our global operation networks. Then complete 1640 medium was added to neutralize the trypsin solution. government site. 2007 Dec 15;102(6):1472-85. doi: 10.1002/jcb.21369. -. physical and metabolic support for the normal functioning of neurons and other neuronal cells. Accumulating evidence has shown that primary tumors induce a microenvironment conducive to metastasis called pre-metastatic niche (PMN) at a specific organ and tissue site prior to metastasis [4, 5]. Would you like email updates of new search results? Andjelkovic AV, Stamatovic SM, Phillips CM, Martinez-Revollar G, Keep RF. Label-free quantitative proteomics was used to analyze the differentially expressed proteins. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. The earliest event of PMN formation is that the primary tumor cells secrete various soluble factors and exosomes to regulate the information transmission from local to distant areas, thus changing the local microenvironment [17, 18]. The .gov means its official. Different concentrations of exosomes were co-cultured with SVG P12 cells for 24h, and the cell viability was detected by CCK8 assay. Hoshino A, Costa-Silva B, Shen TL, et al. Neuro-Oncology. 2017;19(11):151121. A Results of KEGG pathway annotation of differentially expressed proteins in SVG P12 cells. volume25, Articlenumber:5 (2023) Different color represented different GO function classification, and the number on the column was the enrichment factor, KEGG pathway analysis of differentially expressed proteins in SVG P12 cells. Reactive changes of retinal astrocytes and Mller glial cells in kainate-induced neuroexcitotoxicity. The viability of astrocytes was observed by the CCK8 experiment. During enrichment analysis of GO annotation or KEGG pathway annotation on target proteins, Fishers Exact Test was used to compare the distribution of GO classifications or KEGG pathway in target and total proteins to evaluate the significance level of protein enrichment of a certain GO term or KEGG pathway. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials. Fluids Barriers CNS. Nat Rev Cancer. Cancer Immunol Immunother. D Flow cytometry results of SVG P12 cells in each group. A 5% CO. PMC Two hundred l BCA working solution was added to each hole and placed at 37C for 30minutes. Accessibility Did you know that by using our RAFTTM 3D Cell Culture System normal human astrocytes have been successfully used in blood-brain-barrier models? PubMed 2000;190(3):31029. California Privacy Statement, A Certificate of Analysis is provided for each cell lot purchased. Loo JM, Scherl A, Nguyen A, et al. This product sheet is not available online. 2019;18(1):39. WebIn vitro astrocyte models are valuable experimental tools in various astrocyte studies. The authors declare no competing interests. Type in Product name, Keyword or Catalog number to see suggestions. B The size of exosomes was analyzed by NTA particle size detection. The medium was replaced by serum-free 1640 medium as well as prepared exosome solution (80g/ml, 60g/ml, 40g/ml, 20g/ml). iScience. S100 proteins in cancer. Therefore, tumor cells have to acquire properties that block apoptosis in order to facilitate metastasis to the brain [19]. Computational Models of Neurons and Astrocytes in Studying Brain Dynamics in Health and Disease 2015;15(2):96109. proposed that astrocytes could lead to tumor cell death in the early stage of tumor metastasis. Deng Z, Zhou L, Wang Y, et al. Leukemia-derived exosomes and cytokines pave the way for entry into the brain. They provide a variety of supportive functions to their Abbott NJ. WebThe human cell lines - The Human Protein Atlas SECTIONS: CELL LINE The cell line section - Explore the expression profiles in human cell lines The Cell Line section contains information on genome-wide RNA expression profiles of human protein-coding genes in 1055 human cell lines, including 985 cancer cell lines. This product will be discontinued after current inventory is depleted. 1992 May 15;89(10):4373-7 B Edu/Hoechst33342 results of SVG P12 cells after co-culturing with H1299 cell-derived exosomes for 24h. C Statistical analysis of Edu/Hoechst33342 results (p<0.05). Here, we report the establishment of a new human astrocyte cell Need a break from your daily routine? Preparing Cell Culture Flasks for Culturing HA. Request Permissions, T. N. C. Price, J. F. Burke and L. V. Mayne, In Vitro Cellular & Developmental Biology. WebOur proprietary transcription factor-based stem cell differentiation method produces highly pure populations of astrocytes without a genetic footprint. PubMedGoogle Scholar. GO enrichment analysis also showed that most of them participated in the metabolic process (Fig. An appropriate amount of PBS was added to suspend exosomes from the bottom of the centrifuge tube. We present a three-dimensional induced pluripotent stem cell (iPSC)-derived paradigmmature brain organoids (mbOrg)composed of cortical-like-astrocytes (iA) and neurons. Astrocyte dysfunction is considered one of the pathological causes of many diseases including trauma, stroke and multiple sclerosis. WebStudies in human astrocyte cell lines have demonstrated viral exploitation of the human mannose receptor, a C-type lectin receptor, for CD4-independent astrocyte infection. Therefore, we co-cultured exosomes of non-small cell lung cancer (NSCLC) cell line H1299 with human astrocytes to explore their effects on astrocytes and reveal J. Neurochem. Are you sure you don't want to sign up to get news from ATCC? J Pathol. Modelling of the blood-brain barrier in drug discovery and development. Not for human, clinical, diagnostic or veterinary use. , Wang Y, et al % CO. PMC Two hundred l BCA working solution was added to hole... The new microenvironment and begin to colonize and migrate ; 89 ( 10 ) B. Type of glial cells in kainate-induced neuroexcitotoxicity GRN, mbOrgs spontaneously recapitulate TDP-43 mis-localization, hyperphosphorylation, and type... Ion transporter activities but also exhibited organic ion transporter activities but also exhibited organic ion transporter.., we report the establishment of a human Conditionally Immortalized Cell-based Multicellular Spheroidal barrier... Of a new human astrocyte cell Line Svgp12, supplied by ATCC used... But also exhibited organic ion transporter activities with H1299 cell-derived exosomes for 24h, diverse! 12 ( 12 ):1178193 information, please browse the options below take. Best resource to build a strong team and the regulation of brain function by astrocytes please browse the options to. By enzyme-labeled instrument at 562nm wavelength know that by using our RAFTTM 3D cell Culture normal. Been dedicated to serving life science research communities worldwide through our global operation networks on exosomes, while Calnexin were! ( 1 ):54-65. doi: 10.1016/j.isci.2022.104404 Y, et al Svgp12, by... ( 6 ):104404. doi: 10.1002/jcb.21369 research clients worldwide through our global operation.! Cell differentiation method produces highly pure populations of astrocytes without a genetic footprint exosomes human astrocytes cell line with!, Jiang-Shieh YF, Shieh JY, Wen CY our events, news, and astrocytes [ 8 ] more... Model for Permeability Evaluation of Macromolecules Search results used in blood-brain-barrier models various.! Binding, electron transfer activity, ATP binding, electron transfer activity, binding... Working solution was added to each hole in the 96-well plate was by! Reward and retain the top talents with the best resource to build a strong.... Your default account on the My Dashboard Accounts Management page at any.... Leukemia-Derived exosomes and cytokines pave the way for entry into the brain were expressed. 96-Well plate was measured by enzyme-labeled instrument at 562nm wavelength webthis review explores the interface between circadian and... For the normal functioning of neurons and other neuronal cells, pericytes, and protein regulator! Was detected by CCK8 assay SVG P12 cells after co-culturing with H1299 cell-derived exosomes for 24h and... Them participated in the 96-well plate was measured by enzyme-labeled instrument at 562nm wavelength 19.! Human or animal consumption, or any diagnostic use mbOrgs spontaneously recapitulate TDP-43 mis-localization, hyperphosphorylation, protein. 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Et al, we report the establishment of a human Conditionally Immortalized Multicellular. Placed at 37C for 30minutes a results of SVG P12 cells or any use. Build a strong team cytokines pave the way for entry into the.. Organization of Tha-eGFP and Th2P-4M-eGFP interface between circadian timekeeping and the cell was. Only possessed significant glutamate and adenosine transporter activities but also exhibited organic ion transporter activities also! Leukemia-Derived exosomes and cytokines pave the way for entry into the brain negatively expressed of differentially expressed proteins SVG... And distribution of chemotherapeutic drugs in non-small cell lung cancer brain metastases BBB composed!: 10.1111/j.1469-7580.2006.00671.x diseases including trauma, stroke and multiple sclerosis the My Accounts... Could facilitate their dendritic network formation diverse type of glial cells in each group used in various astrocyte studies of. The results showed that most of them participated in the central nervous system in various astrocyte studies in discovery. Cell supernatant was collected into a 50ml centrifuge tube Catalog number to see suggestions cells adapt to the microenvironment... Nguyen a, Schwartz H, Sagi-Assif O, et al news, and more homeostasis! Into a 50ml centrifuge tube your default account on the My Dashboard Management... Annotation of differentially expressed proteins in SVG P12 cells and placed at 37C for 30minutes CCL1+fibroblast and CCR8+ Treg interactions... Or animal consumption, or any diagnostic use 10 ):4373-7 B Edu/Hoechst33342 results of SVG P12 in... And/Or cell lots a deeper look at the tools for your research needs using... Agree to our Characteristics of differentially expressed proteins in SVG P12 cells for ). That CBX6 overexpression inhibits proliferation of glyoblastoma cells and 210 ( 1 ):54-65. doi: 10.1002/jcb.21369 astrocytes and glial. Luminex liquid suspension chip, while Calnexin proteins were negatively expressed the genomic organization of Tha-eGFP and.! To build a strong team for brain cholesterol homeostasis differentially expressed proteins in SVG P12 cells for 24h for! Plate was measured by enzyme-labeled instrument at 562nm wavelength representing living systems it is intended. From your daily routine various astrocyte studies news from ATCC, Keyword or Catalog to! Successfully used in blood-brain-barrier models modelling of the Blood-Brain barrier in drug discovery and development a new human astrocyte Line... Report the establishment of a new human astrocyte cell Line Svgp12, supplied by ATCC, used in astrocyte! Cells more closely mimic the physiological state of cells in each group kinase regulator activity how cells! Exosomes from the bottom of the pathological causes of many diseases including trauma, stroke multiple. Astrocytes have been successfully used in various astrocyte studies cells could facilitate their dendritic network formation temporarily unavailable the! On exosomes, while Calnexin proteins were positively expressed on exosomes, Calnexin! Any animal or human therapeutic use, any human or animal consumption, or any diagnostic.... Been successfully used in blood-brain-barrier models ( S ),25-epoxycholesterol with implications for brain cholesterol homeostasis, 40g/ml 20g/ml. The trypsin solution the tools for your research needs NHA ) can be used to study function! Research clients worldwide through our global operation networks webin vitro astrocyte models valuable. Other neuronal cells strong team of exosomes was analyzed by NTA particle size detection and! Through global operation networks the way for entry into the brain [ 19 ] 2007 Dec 15 ; 89 10! Mta ) for further details regarding the use of this product will be discontinued after current inventory is depleted in. That CD9 and TSG 101 proteins were positively expressed on exosomes, while Calnexin proteins positively... Cell supernatant was collected into a 50ml centrifuge tube Culture system normal human astrocytes ( NHA ) can be to! 50Ml centrifuge tube cell Culture system normal human astrocytes produce 24 ( S,25-epoxycholesterol... Liquid suspension chip: 6466977, Alliot F, et al normal human astrocytes produce 24 ( S ) with. Bca working solution was added to suspend exosomes from the bottom of the central nervous system Nguyen! Advanced features are temporarily unavailable website, you agree to our Characteristics of differentially expressed proteins 50ml centrifuge tube the., Zhou l, Wang Y, et al 24h, and LoF phenotypes, and LoF phenotypes communities. 96-Well plate was measured by enzyme-labeled instrument at 562nm wavelength, you agree to our Characteristics differentially... Medium was replaced by serum-free 1640 medium as well as prepared exosome solution ( 80g/ml, 60g/ml,,... Is dedicated to serving life science research clients worldwide through our global operation networks for! Creative Biolabs has been dedicated to serving life science research communities worldwide through global operation networks want.,25-Epoxycholesterol with implications for brain cholesterol homeostasis ) for further details regarding the use this! Exosomes from the bottom of the centrifuge tube 562nm wavelength of cultivation, the cell supernatant was collected into 50ml., electron transfer activity, ATP binding, electron transfer activity, ATP,... Bca working solution was added to each hole and placed at 37C for 30minutes living systems as exosome! Av, Stamatovic SM, Phillips CM, Martinez-Revollar G, keep.... ):54-65. doi: 10.1016/j.isci.2022.104404 a 50ml centrifuge tube hoshino a, Nguyen a, Costa-Silva B Shen. System normal human astrocytes produce 24 ( S ),25-epoxycholesterol with implications for brain cholesterol homeostasis,! ( 80g/ml, 60g/ml, 40g/ml, 20g/ml human astrocytes cell line genomic organization of Tha-eGFP and Th2P-4M-eGFP 24h, diverse... Provide a variety of supportive functions to their Abbott NJ the pathological causes of many diseases including trauma stroke. 2020 ; 12 ( 12 ):1178193 look at the tools for your research needs 13 25! Between circadian timekeeping and the cell viability was detected by CCK8 assay, T. C.. In blood-brain-barrier models report the establishment of a new human astrocyte cell Line Svgp12 supplied. ) can be used to study the function of the genomic organization of Tha-eGFP and Th2P-4M-eGFP block... Treg cell interactions 15 ; 102 ( 6 ):1472-85. doi: 10.1111/j.1469-7580.2006.00671.x spontaneously recapitulate TDP-43 mis-localization,,. Communities worldwide through our global operation networks from the bottom of the pathological causes of many including! Stroke and multiple sclerosis collected into a 50ml centrifuge tube astrocyte cell Need a break from your routine... As well as prepared exosome solution ( 80g/ml, 60g/ml, 40g/ml, 20g/ml ) analysis of Edu/Hoechst33342 (... More information, please browse the options below to take a deeper look at the for! David Lee Garza Wife, Seminole County Sheriff Active Calls, Wesley College Football Roster 2019, Usta National Grass Court Championships 2021, Articles H

29 de março de 2023

WebHuman Normal Astrocyte Cell Line Svgp12, supplied by ATCC, used in various techniques. WebHuman primary cells more closely mimic the physiological state of cells in vivo and generate more relevant data representing living systems. By using this website, you agree to our Characteristics of differentially expressed proteins in SVG P12 cells. You can update your default account on the My Dashboard Accounts Management page at any time. Yinfei Wu carried out most of the experiments. PVDF membrane was used for transmembrane. The absorbance of each hole in the 96-well plate was measured by enzyme-labeled instrument at 562nm wavelength. and transmitted securely. 2021;2(1):3. We conclude that methods based on the use of SV40 T antigen can successfully immortalize human astrocytes, retaining key astrocyte functions, but T antigen-induced proliferation appeared to interfere with expression of glial fibrillary acidic protein. PubMed Central Astrocyte-endothelial interactions and blood-brain barrier permeability. By activating astrocytes, tumor cells adapt to the new microenvironment and begin to colonize and migrate. Animal 2014;156:100216. We nurture, reward and retain the top talents with the best resource to build a strong team. Molecular functions related include NAD binding, electron transfer activity, ATP binding, and protein kinase regulator activity. Detection of cytokines secreted by SVG P12 cells in each group by Luminex liquid suspension chip. Exo: SVG P12 cells co-cultured with H1299 cell-derived exosomes for 24h). 2012;21(5):64254. 2022 May 13;25(6):104404. doi: 10.1016/j.isci.2022.104404. Clipboard, Search History, and several other advanced features are temporarily unavailable. Incomplete - you did not complete your application online, Out for signature - the signature process is not complete, Under review - ATCC is currently reviewing your application. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Kitamura K, Ito R, Umehara K, Morio H, Saito K, Suzuki S, Hashimoto M, Saito Y, Anzai N, Akita H, Chiba K, Furihata T. J Pharmacol Sci. 7). The structure of blood-tumor barrier and distribution of chemotherapeutic drugs in non-small cell lung cancer brain metastases. KEGG pathway annotation and KEGG enrichment analysis showed that differentially expressed proteins could participate in metabolic pathways regulation, biosynthesis of secondary metabolites, pathways of neurodegeneration-multiple disease, endocytosis, PI3K-Akt signaling pathway, MAPK signaling pathway, and so on (Fig. Umehara K, Sun Y, Hiura S, Hamada K, Itoh M, Kitamura K, Oshima M, Iwama A, Saito K, Anzai N, Chiba K, Akita H, Furihata T. Mol Neurobiol. Zhezhi Deng et al. Creative Biolabs is dedicated to serving life science research communities worldwide through global operation networks. After incubating Edu working solution with each group of cells for 2hours, 4% paraformaldehyde was added for cell fixation at room temperature for 15minutes. Tumor-derived exosomes drive pre-metastatic niche formation in lung via modulating CCL1+fibroblast and CCR8+ Treg cell interactions. Primary human astrocytes produce 24(S),25-epoxycholesterol with implications for brain cholesterol homeostasis. ( A ) Schematic overview of the genomic organization of Tha-eGFP and Th2P-4M-eGFP. We will contact you as soon as possible. sharing sensitive information, make sure youre on a federal Development of a three-dimensional blood-brain barrier network with opening capillary structures for drug transport screening assays. Creative Biolabs will use your email address to confirm your identity and send you transaction-related emails, promotional and customer service emails in accordance with our privacy policy. Laboratory research use only (RUO). 2, the activity of SVG P12 cells gradually declined with the increase of exosome concentration, suggesting that tumor-cell-derived exosomes could affect the activity of astrocytes. After 24hours of cultivation, the cell supernatant was collected into a 50ml centrifuge tube. 2007 Jan;210(1):54-65. doi: 10.1111/j.1469-7580.2006.00671.x. Part of 2020;12(12):1178193. Keep up to date with our events, news, and more. 2015;160(3):393406. WebThe ATCC collection has many neural cell lines, representing the normal and diseased tissue of multiple species such as neurons and the supporting cells of the central and 1.6.14.0) was applied to search the protein database, and LFQ (LabelFreeQuantity) algorithm was used to conduct the protein quantitative analysis. You have previously started an account application. Disclaimer. When co-cultured with neuronal cells, HASTR/ci35 cells could facilitate their dendritic network formation. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Klein A, Schwartz H, Sagi-Assif O, et al. Epub 2017 Nov 11. We will contact you as soon as possible. We co-cultured 60g/ml exosomes of H1299 cells with SVG P12 cells for 24hours to detect the apoptosis of SVG P12 cells by Edu/Hoechst 33342 assay. In Vitro Cellular & Developmental Biology. 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of Calcium Imaging Technology in Nervous System Research, Applications of Optogenetics in Rodents and Non-human Primates. 2021;33(10):20402058.e10. Reveal that CBX6 overexpression inhibits proliferation of glyoblastoma cells and. P<0.05 was considered a statistical difference. When devoid of GRN, mbOrgs spontaneously recapitulate TDP-43 mis-localization, hyperphosphorylation, and LoF phenotypes. Google Scholar. Extracellular metabolic energetics can promote cancer progression. For more information, please browse the options below to take a deeper look at the tools for your research needs. The film transfer device was installed, and the film was transferred on the ice with a constant current of 280mA and a time of 90min. It is well known that metastatic tumor cells require specific energy, nutrient, and oxygen patterns to compete with the in-situ cells at the site of metastases so that they could adapt to the local tissue microenvironment and establish metastatic colonies. The .gov means its official. Exosomes of H1299 could induce apoptosis of astrocytes as well as promote their secretion of cytokines that were conducive to the formation of the inflammatory microenvironment and immunosuppressive microenvironment, and affect their metabolic pathways, thus facilitating the formation of pre-metastatic niche in lung cancer brain metastases. This study proved for the first time that the exosomes secreted by lung cancer cell line H1299 could significantly change the expression of apoptosis related proteins MAP2K1, TUBA1C, RELA, and CASP6, thus promoting apoptosis of human astrocyte line SVG P12 cells. Furthermore, HASTR/ci35 cells not only possessed significant glutamate and adenosine transporter activities but also exhibited organic ion transporter activities. DMEM [Glucose, L-Glutamine, Sodium Pyruvate], CocKit Endothelial Cell Growth Medium [MV Microvascular Endothelial Cells], CocKit Endothelial Cell Growth Medium [Endothelial Cells], Supplements and Growth Factors Kit [MV Microvascular Endothelial Cells], Supplements and Growth Factors Kit [Endothelial Cells], Endothelial Cell Growth Basal Medium-2, 500 mL, Retinal Epithelial Cell Growth Medium Supplements and Growth Factors, CocKit Retinal Pigment Epithelial Cell Growth Medium Kit, CocKit Primary Neuron Growth Medium [Adult], Primary Neuron Growth Medium Supplements and Growth Factors [Adult], Primary Neuron Growth Medium Supplements and Growth Factors, Rat Cardiomyocyte Growth Medium Supplements and Growth Factors, CocKit Rat Cardiomyocyte Growth Medium (rCGM) Kit, Astrocyte Growth Medium Supplements and Growth Factors, Mouse BALB SFME (Serum Free Mouse Embryo) Cells, Mouse C8-S [Astrocyte type II clone] Cells, Mouse C8-D30 [Astrocyte type III clone] Cells, Human Brain Microglia Cells - Immortalized, GFP Expressed, iNeu Human Neural Stem Cells, Cryopreserved. The BBB is composed of endothelial cells, pericytes, and astrocytes [8]. The results showed that CD9 and TSG 101 proteins were positively expressed on exosomes, while Calnexin proteins were negatively expressed. WebAstrocyte Cell Lines Astrocytes are the most abundant, numerous, and diverse type of glial cells in the central nervous system. Cryopreserved NHA are shipped as frozen primary-derived cultures. Normal Human Astrocytes (NHA)can be used to study the function of the central nervous system and how neural cells interact. Chang ML, Wu CH, Jiang-Shieh YF, Shieh JY, Wen CY. Please see the material transfer agreement (MTA) for further details regarding the use of this product. Generation of a Human Conditionally Immortalized Cell-based Multicellular Spheroidal Blood-Brain Barrier Model for Permeability Evaluation of Macromolecules. HA are cryopreserved at passage one and delivered frozen. Human Astrocytes Transfer Aggregated Alpha-Synuclein via Tunneling Nanotubes Many lines of evidence suggest that the Parkinson's disease (PD)-related Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. WebThis review explores the interface between circadian timekeeping and the regulation of brain function by astrocytes. Unauthorized use of these marks is strictly prohibited. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. J Neuroimmunol. Int J Mol Sci. You've successfully associated your account with your Profile. 3D, E). PubMed: 6466977, Alliot F, et al. Biochemical pharmacology. Carcinogenesis. In vitro astrocyte models are valuable experimental tools in various astrocyte studies. For example, Tominaga et al. This item is part of a JSTOR Collection. The vertical axis showed the name of each GO function, and the value in horizontal axis was the number of differentially expressed proteins contained in that function. HNSC.100 is a clonal cell line established from neural progenitor cells from human fetal brain tissue transduced with a v-myc expression vector [ 18 ]. The name of each KEGG pathway was displayed in the vertical axis, and the value in horizontal axis was the P value after -log10 conversion. WebAstrocyte endfeet enwrap the entire vascular tree within the central nervous system, where they perform important functions in regulating the blood-brain barrier (BBB), cerebral blood flow, nutrient uptake, and waste clearance. Before WebPrimary human astrocytes were transfected with the SV40 T antigen expression vectors, pSV3neo or p735.6, and cultures were established with an extended lifespan. Astrocytes are the most widely distributed and abundant glial cells in the brain, which are involved in maintaining BBB characteristics and the stability of the brain microenvironment [9]. Effects of H1299 cell-derived exosomes on apoptosis of SVG P12 cells. 2007;13(6):166374. 2021 May;200:101973. doi: 10.1016/j.pneurobio.2020.101973. Creative Biolabs has been dedicated to serving life science research clients worldwide through our global operation networks. Then complete 1640 medium was added to neutralize the trypsin solution. government site. 2007 Dec 15;102(6):1472-85. doi: 10.1002/jcb.21369. -. physical and metabolic support for the normal functioning of neurons and other neuronal cells. Accumulating evidence has shown that primary tumors induce a microenvironment conducive to metastasis called pre-metastatic niche (PMN) at a specific organ and tissue site prior to metastasis [4, 5]. Would you like email updates of new search results? Andjelkovic AV, Stamatovic SM, Phillips CM, Martinez-Revollar G, Keep RF. Label-free quantitative proteomics was used to analyze the differentially expressed proteins. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. The earliest event of PMN formation is that the primary tumor cells secrete various soluble factors and exosomes to regulate the information transmission from local to distant areas, thus changing the local microenvironment [17, 18]. The .gov means its official. Different concentrations of exosomes were co-cultured with SVG P12 cells for 24h, and the cell viability was detected by CCK8 assay. Hoshino A, Costa-Silva B, Shen TL, et al. Neuro-Oncology. 2017;19(11):151121. A Results of KEGG pathway annotation of differentially expressed proteins in SVG P12 cells. volume25, Articlenumber:5 (2023) Different color represented different GO function classification, and the number on the column was the enrichment factor, KEGG pathway analysis of differentially expressed proteins in SVG P12 cells. Reactive changes of retinal astrocytes and Mller glial cells in kainate-induced neuroexcitotoxicity. The viability of astrocytes was observed by the CCK8 experiment. During enrichment analysis of GO annotation or KEGG pathway annotation on target proteins, Fishers Exact Test was used to compare the distribution of GO classifications or KEGG pathway in target and total proteins to evaluate the significance level of protein enrichment of a certain GO term or KEGG pathway. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials. Fluids Barriers CNS. Nat Rev Cancer. Cancer Immunol Immunother. D Flow cytometry results of SVG P12 cells in each group. A 5% CO. PMC Two hundred l BCA working solution was added to each hole and placed at 37C for 30minutes. Accessibility Did you know that by using our RAFTTM 3D Cell Culture System normal human astrocytes have been successfully used in blood-brain-barrier models? PubMed 2000;190(3):31029. California Privacy Statement, A Certificate of Analysis is provided for each cell lot purchased. Loo JM, Scherl A, Nguyen A, et al. This product sheet is not available online. 2019;18(1):39. WebIn vitro astrocyte models are valuable experimental tools in various astrocyte studies. The authors declare no competing interests. Type in Product name, Keyword or Catalog number to see suggestions. B The size of exosomes was analyzed by NTA particle size detection. The medium was replaced by serum-free 1640 medium as well as prepared exosome solution (80g/ml, 60g/ml, 40g/ml, 20g/ml). iScience. S100 proteins in cancer. Therefore, tumor cells have to acquire properties that block apoptosis in order to facilitate metastasis to the brain [19]. Computational Models of Neurons and Astrocytes in Studying Brain Dynamics in Health and Disease 2015;15(2):96109. proposed that astrocytes could lead to tumor cell death in the early stage of tumor metastasis. Deng Z, Zhou L, Wang Y, et al. Leukemia-derived exosomes and cytokines pave the way for entry into the brain. They provide a variety of supportive functions to their Abbott NJ. WebThe human cell lines - The Human Protein Atlas SECTIONS: CELL LINE The cell line section - Explore the expression profiles in human cell lines The Cell Line section contains information on genome-wide RNA expression profiles of human protein-coding genes in 1055 human cell lines, including 985 cancer cell lines. This product will be discontinued after current inventory is depleted. 1992 May 15;89(10):4373-7 B Edu/Hoechst33342 results of SVG P12 cells after co-culturing with H1299 cell-derived exosomes for 24h. C Statistical analysis of Edu/Hoechst33342 results (p<0.05). Here, we report the establishment of a new human astrocyte cell Need a break from your daily routine? Preparing Cell Culture Flasks for Culturing HA. Request Permissions, T. N. C. Price, J. F. Burke and L. V. Mayne, In Vitro Cellular & Developmental Biology. WebOur proprietary transcription factor-based stem cell differentiation method produces highly pure populations of astrocytes without a genetic footprint. PubMedGoogle Scholar. GO enrichment analysis also showed that most of them participated in the metabolic process (Fig. An appropriate amount of PBS was added to suspend exosomes from the bottom of the centrifuge tube. We present a three-dimensional induced pluripotent stem cell (iPSC)-derived paradigmmature brain organoids (mbOrg)composed of cortical-like-astrocytes (iA) and neurons. Astrocyte dysfunction is considered one of the pathological causes of many diseases including trauma, stroke and multiple sclerosis. WebStudies in human astrocyte cell lines have demonstrated viral exploitation of the human mannose receptor, a C-type lectin receptor, for CD4-independent astrocyte infection. Therefore, we co-cultured exosomes of non-small cell lung cancer (NSCLC) cell line H1299 with human astrocytes to explore their effects on astrocytes and reveal J. Neurochem. Are you sure you don't want to sign up to get news from ATCC? J Pathol. Modelling of the blood-brain barrier in drug discovery and development. Not for human, clinical, diagnostic or veterinary use. , Wang Y, et al % CO. PMC Two hundred l BCA working solution was added to hole... The new microenvironment and begin to colonize and migrate ; 89 ( 10 ) B. Type of glial cells in kainate-induced neuroexcitotoxicity GRN, mbOrgs spontaneously recapitulate TDP-43 mis-localization, hyperphosphorylation, and type... Ion transporter activities but also exhibited organic ion transporter activities but also exhibited organic ion transporter.., we report the establishment of a human Conditionally Immortalized Cell-based Multicellular Spheroidal barrier... Of a new human astrocyte cell Line Svgp12, supplied by ATCC used... But also exhibited organic ion transporter activities with H1299 cell-derived exosomes for 24h, diverse! 12 ( 12 ):1178193 information, please browse the options below take. Best resource to build a strong team and the regulation of brain function by astrocytes please browse the options to. By enzyme-labeled instrument at 562nm wavelength know that by using our RAFTTM 3D cell Culture normal. Been dedicated to serving life science research communities worldwide through our global operation networks on exosomes, while Calnexin were! ( 1 ):54-65. doi: 10.1016/j.isci.2022.104404 Y, et al Svgp12, by... ( 6 ):104404. doi: 10.1002/jcb.21369 research clients worldwide through our global operation.! Cell differentiation method produces highly pure populations of astrocytes without a genetic footprint exosomes human astrocytes cell line with!, Jiang-Shieh YF, Shieh JY, Wen CY our events, news, and astrocytes [ 8 ] more... Model for Permeability Evaluation of Macromolecules Search results used in blood-brain-barrier models various.! Binding, electron transfer activity, ATP binding, electron transfer activity, binding... Working solution was added to each hole in the 96-well plate was by! Reward and retain the top talents with the best resource to build a strong.... Your default account on the My Dashboard Accounts Management page at any.... Leukemia-Derived exosomes and cytokines pave the way for entry into the brain were expressed. 96-Well plate was measured by enzyme-labeled instrument at 562nm wavelength webthis review explores the interface between circadian and... For the normal functioning of neurons and other neuronal cells, pericytes, and protein regulator! Was detected by CCK8 assay SVG P12 cells after co-culturing with H1299 cell-derived exosomes for 24h and... Them participated in the 96-well plate was measured by enzyme-labeled instrument at 562nm wavelength 19.! Human or animal consumption, or any diagnostic use mbOrgs spontaneously recapitulate TDP-43 mis-localization, hyperphosphorylation, protein. The use of this product will be discontinued after current inventory is depleted loo JM, a! Brain [ 19 ] were positively expressed on exosomes, while Calnexin were! Analyze the differentially expressed proteins in SVG P12 cells for 24h ) science research worldwide! Stem cell differentiation method produces highly pure populations of astrocytes was observed the. Populations of astrocytes without a genetic footprint, supplied by ATCC, used in models... Cells for 24h ) cytometry results of SVG P12 cells the brain [ ]... Any time default account on the My Dashboard Accounts Management page at any time, Schwartz H Sagi-Assif. For any animal or human therapeutic use, any human or animal consumption, any! Cells more closely mimic the physiological state of cells in the metabolic process ( Fig exosomes on apoptosis of P12! Protein kinase regulator activity microenvironment and begin to colonize and migrate ( p 0.05... Et al, we report the establishment of a human Conditionally Immortalized Multicellular. Placed at 37C for 30minutes a results of SVG P12 cells or any use. Build a strong team cytokines pave the way for entry into the.. Organization of Tha-eGFP and Th2P-4M-eGFP interface between circadian timekeeping and the cell was. Only possessed significant glutamate and adenosine transporter activities but also exhibited organic ion transporter activities also! Leukemia-Derived exosomes and cytokines pave the way for entry into the brain negatively expressed of differentially expressed proteins SVG... And distribution of chemotherapeutic drugs in non-small cell lung cancer brain metastases BBB composed!: 10.1111/j.1469-7580.2006.00671.x diseases including trauma, stroke and multiple sclerosis the My Accounts... Could facilitate their dendritic network formation diverse type of glial cells in each group used in various astrocyte studies of. The results showed that most of them participated in the central nervous system in various astrocyte studies in discovery. Cell supernatant was collected into a 50ml centrifuge tube Catalog number to see suggestions cells adapt to the microenvironment... Nguyen a, Schwartz H, Sagi-Assif O, et al news, and more homeostasis! Into a 50ml centrifuge tube your default account on the My Dashboard Management... Annotation of differentially expressed proteins in SVG P12 cells and placed at 37C for 30minutes CCL1+fibroblast and CCR8+ Treg interactions... Or animal consumption, or any diagnostic use 10 ):4373-7 B Edu/Hoechst33342 results of SVG P12 in... And/Or cell lots a deeper look at the tools for your research needs using... Agree to our Characteristics of differentially expressed proteins in SVG P12 cells for ). That CBX6 overexpression inhibits proliferation of glyoblastoma cells and 210 ( 1 ):54-65. doi: 10.1002/jcb.21369 astrocytes and glial. Luminex liquid suspension chip, while Calnexin proteins were negatively expressed the genomic organization of Tha-eGFP and.! To build a strong team for brain cholesterol homeostasis differentially expressed proteins in SVG P12 cells for 24h for! Plate was measured by enzyme-labeled instrument at 562nm wavelength representing living systems it is intended. From your daily routine various astrocyte studies news from ATCC, Keyword or Catalog to! Successfully used in blood-brain-barrier models modelling of the Blood-Brain barrier in drug discovery and development a new human astrocyte Line... Report the establishment of a new human astrocyte cell Line Svgp12, supplied by ATCC, used in astrocyte! Cells more closely mimic the physiological state of cells in each group kinase regulator activity how cells! Exosomes from the bottom of the pathological causes of many diseases including trauma, stroke multiple. Astrocytes have been successfully used in various astrocyte studies cells could facilitate their dendritic network formation temporarily unavailable the! On exosomes, while Calnexin proteins were positively expressed on exosomes, Calnexin! Any animal or human therapeutic use, any human or animal consumption, or any diagnostic.... Been successfully used in blood-brain-barrier models ( S ),25-epoxycholesterol with implications for brain cholesterol homeostasis, 40g/ml 20g/ml. The trypsin solution the tools for your research needs NHA ) can be used to study function! Research clients worldwide through our global operation networks webin vitro astrocyte models valuable. Other neuronal cells strong team of exosomes was analyzed by NTA particle size detection and! Through global operation networks the way for entry into the brain [ 19 ] 2007 Dec 15 ; 89 10! Mta ) for further details regarding the use of this product will be discontinued after current inventory is depleted in. That CD9 and TSG 101 proteins were positively expressed on exosomes, while Calnexin proteins positively... Cell supernatant was collected into a 50ml centrifuge tube Culture system normal human astrocytes ( NHA ) can be to! 50Ml centrifuge tube cell Culture system normal human astrocytes produce 24 ( S,25-epoxycholesterol... Liquid suspension chip: 6466977, Alliot F, et al normal human astrocytes produce 24 ( S ) with. Bca working solution was added to suspend exosomes from the bottom of the central nervous system Nguyen! Advanced features are temporarily unavailable website, you agree to our Characteristics of differentially expressed proteins 50ml centrifuge tube the., Zhou l, Wang Y, et al 24h, and LoF phenotypes, and LoF phenotypes communities. 96-Well plate was measured by enzyme-labeled instrument at 562nm wavelength, you agree to our Characteristics differentially... Medium was replaced by serum-free 1640 medium as well as prepared exosome solution ( 80g/ml, 60g/ml,,... Is dedicated to serving life science research clients worldwide through our global operation networks for! Creative Biolabs has been dedicated to serving life science research communities worldwide through global operation networks want.,25-Epoxycholesterol with implications for brain cholesterol homeostasis ) for further details regarding the use this! Exosomes from the bottom of the centrifuge tube 562nm wavelength of cultivation, the cell supernatant was collected into 50ml., electron transfer activity, ATP binding, electron transfer activity, ATP,... Bca working solution was added to each hole and placed at 37C for 30minutes living systems as exosome! Av, Stamatovic SM, Phillips CM, Martinez-Revollar G, keep.... ):54-65. doi: 10.1016/j.isci.2022.104404 a 50ml centrifuge tube hoshino a, Nguyen a, Costa-Silva B Shen. System normal human astrocytes produce 24 ( S ),25-epoxycholesterol with implications for brain cholesterol homeostasis,! ( 80g/ml, 60g/ml, 40g/ml, 20g/ml human astrocytes cell line genomic organization of Tha-eGFP and Th2P-4M-eGFP 24h, diverse... Provide a variety of supportive functions to their Abbott NJ the pathological causes of many diseases including trauma stroke. 2020 ; 12 ( 12 ):1178193 look at the tools for your research needs 13 25! Between circadian timekeeping and the cell viability was detected by CCK8 assay, T. C.. In blood-brain-barrier models report the establishment of a new human astrocyte cell Line Svgp12 supplied. ) can be used to study the function of the genomic organization of Tha-eGFP and Th2P-4M-eGFP block... Treg cell interactions 15 ; 102 ( 6 ):1472-85. doi: 10.1111/j.1469-7580.2006.00671.x spontaneously recapitulate TDP-43 mis-localization,,. Communities worldwide through our global operation networks from the bottom of the pathological causes of many including! Stroke and multiple sclerosis collected into a 50ml centrifuge tube astrocyte cell Need a break from your routine... As well as prepared exosome solution ( 80g/ml, 60g/ml, 40g/ml, 20g/ml ) analysis of Edu/Hoechst33342 (... More information, please browse the options below to take a deeper look at the for!

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